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Multilocus Sequence Typing Pulsed

Utility of multilocus sequence typing as an

Multilocus sequence typing (MLST) has been proven useful for the study of the global population structure of Campylobacter jejuni; however,its usefulness for the investigation of outbreaks of disease caused by C.jejuni has not been proven.In this study,MLST plus sequencing of the flaA short variSubject multilocus sequence typing - PubAg Search ResultsWe aimed to investigate genetic relatedness of S.agalactiae strains isolated from humans and animal origins,including cattle and fish,using capsular gene typing,pulsed-field gel electrophoresis (PFGE),and multilocus sequence typing techniques.Standardization of multilocus sequence typing scheme for Oct 01,2013 Multilocus Sequence Typing Pulsed#0183;This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species.A total of 89 clinical isolates of M.abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included.Forty-two isolates were identified as M.abscessus,and 29,as Mycobacterium massiliense

Standardization of multilocus sequence typing scheme for

Oct 01,2013 Multilocus Sequence Typing Pulsed#0183;This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species.A total of 89 clinical isolates of M.abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included.Forty-two isolates were identified as M.abscessus,and 29,as Mycobacterium massilienseResistance and Heteroresistance to Colistin in Pseudomonas Pulsed-field gel electrophoresis and multilocus sequence typing were performed.Lipid A characteristics were determined via matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS).Two resistant isolates and 9 heteroresistant isolates were selected in this study.Substitutions in PmrB were detected in 2 Related searches for Multilocus Sequence Typing Pulsedmultilocus sequence typing mlstmultilocus enzyme electrophoresisSome results are removed in response to a notice of local law requirement.For more information,please see here.12345NextMultilocus Sequence Typing,Pulsed-Field Gel A total of 208 isolates were typed by sequencing the fla short variable region,macrorestriction analysis resolved by pulsed-field gel electrophoresis (PFGE),and multilocus sequence typing (MLST).We observed a high diversity of human strains during a given summer season.

Related searches for Multilocus Sequence Typing Pulsed

multilocus sequence typing mlstmultilocus enzyme electrophoresisPrevious123456NextSubject multilocus sequence typing - PubAg Search ResultsWe aimed to investigate genetic relatedness of S.agalactiae strains isolated from humans and animal origins,including cattle and fish,using capsular gene typing,pulsed-field gel electrophoresis (PFGE),and multilocus sequence typing techniques.Related searches for Multilocus Sequence Typing Pulsedmultilocus sequence typing mlstmultilocus enzyme electrophoresisReal-Time Whole-Genome Sequencing for Surveillance of During 2015-2016,we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool.Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE),the current standard method.A total of 2,74

Rapid and Simple Universal Escherichia coli Genotyping

The performances were compared to those of multilocus sequence typing (MLST),the DiversiLab automated repetitive element palindromic PCR (REP-PCR),pulsed-field gel electrophoresis (PFGE),and whole-genome sequencing (WGS).We found 66 different profiles among the isolates inPopulation Genetic Structure of Listeria monocytogenes A total of 396 strains were selected on the basis of different pulsed-field gel electrophoresis (PFGE) clusters,serotypes,and strain origins and typed by multilocus sequence typing (MLST),and the MLST results were supplemented with MLST data available from Institut Pasteur,representing human and additional food strains from France.Phylogenetic investigation of Edwardsiella tarda with Oct 10,2013 Multilocus Sequence Typing Pulsed#0183;Edwardsiella tarda is the etiologic agent of Edwardsiellosis,a devastating fish disease prevailing in worldwide aquaculture industries.Here we developed the SpeI-based pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) assays to investigate the genetic diversity of Edwardsiella isolates obtained from aquaculture farms and to get insights into the

Necrotizing Fasciitis Caused by Community-Associated

All recovered isolates belonged to the same genotype (multilocus sequence type ST8,pulsed-field type USA300,and staphylococcal cassette chromosome mec type IV [SCCmecIV]) and carried the Panton Multilocus sequence typing of Arcobacter butzleri isolates MLST,multilocus sequence typing; PFGE,pulsedfield gel electrophoresis.The AW coefficient concerning the unidirectional concordance between PFGE and MLST (AW 0720) was significantly higher ( P = 0049) than that between MLST and PFGE (AW 0532) However,both methods showed a good concordance (i.e.AW Multilocus Sequence Typing Pulsedgt; 050) (Table 5 ).Multilocus sequence typing lacks the discriminatory Comparison of multilocus sequence typing,pulsed-field gel electrophoresis,and antimicrobial susceptibility typing for characterization of Salmonella enterica serotype Newport isolates.Harbottle H,White DG,McDermott PF,Walker RD,Zhao S.J Clin Microbiol,44(7):2449-2457,01 Jul 2006

Multilocus sequence typing (and phylogenetic analysis) of

The molecular epidemiology of C.jejuni and C.coli clinical strains isolated from children with gastroenteritis,was investigated using the multilocus sequence typing method (MLST).This analysis establishes for the first time in Greece and constitutes an important tool for the epidemiological surveillance and control of Campylobacter infection in our country.Multilocus Sequence Typing,Pulsed-Field Gel analysis resolved by pulsed-eld gel electrophoresis (PFGE),and multilocus sequence typing (MLST).We observed a high diversity of human strains during a given summer season.Poultry and human isolates had a higher diversity of sequence types than isolates of bovine origin,for which clonal complexes CC21 (41.6%) and CC61 (18.7%) were Multilocus Sequence Typing for Candida albicans Isolates Multilocus sequence typing (MLST),which is based on DNA sequence analysis of nucleotide polymorphisms within housekeeping gene fragments,is a reliable,objective,and highly discriminating molecular typing method for the characterization of clinical C.albicans isolates [6,7].

Multilocus Sequence Typing and Pulsed Field Gel

Molecular epidemiology multilocus sequence typing pulsed field gel electrophoresis eBURST Streptococcus pneumoniae Haemophilus influenzae Moraxella catarrhalis otitis media This is a preview of subscription content,log in to check access.Multilocus Sequence Typing and Pulsed Field Gel Molecular epidemiology multilocus sequence typing pulsed field gel electrophoresis eBURST Streptococcus pneumoniae Haemophilus influenzae Moraxella catarrhalis otitis media This is a preview of subscription content,log in to check access.Multilocus Sequence Typing Scheme versus Pulsed-Field Gel Aug 01,2014 Multilocus Sequence Typing Pulsed#0183;Pulsed-field gel electrophoresis (PFGE) patterns and dendrograms prepared using the BioNumerics program v.5.1 and the corresponding multilocus sequence typing (MLST) alleles and sequence types (STs) from isolates collected during the five outbreaks.(A) Outbreak 1,mesotherapy (Bel Multilocus Sequence Typing Pulsed#233;m,PA,2004).

Multilocus Sequence Typing Has Better Discriminatory

Multilocus Sequence Typing Has Better Discriminatory Ability for Typing Vibrio cholerae than Does Pulsed-Field Gel Electrophoresis and Provides a Measure of Phylogenetic Relatedness Mamuka Kotetishvili,1,2 O.Colin Stine,1Yuansha Chen,Arnold Kreger,1 Alexander Sulakvelidze,1 Shanmuga Sozhamannan,1 and J.Glenn Morris,Jr.*Multilocus Sequence Typing - an overview ScienceDirect Tanis C.Dingle,Duncan R.MacCannell,in Methods in Microbiology,2015.2.3.1.1 Multilocus sequence typing.Multilocus sequence typing (MLST) typically refers to the systematic sequencing of six or seven well-conserved,house-keeping genes or loci within the bacterial genome.Allelic variation at each locus is catalogued,and a sequence type or lineage is assigned by comparing the set of Molecular and comparative analysis of Salmonella enterica The molecular profiles of all isolates were determined using Pulsed Field Gel Electrophoresis (PFGE) and the sequence types of the strains were obtained using Multi-Locus Sequence Type (MLST) analysis based on amplification and sequence interrogation of seven housekeeping genes (aroC,dnaN,hemD,hisD,purE,sucA,and thrA).

Molecular Typing of Selected Enterococcus faecalis

The present study compared the recently developed multilocus sequence typing (MLST) approach with a well-established molecular typing technique,pulsed-field gel electrophoresis (PFGE),for subspecies differentiation of Enterococcus faecalis isolates.We sequenced intragenic regions of three E.faecalis antigen-encoding genes ( ace ,encoding a collagen and laminin adhesin; efaA ,encoding an Molecular Typing of Clones - Emory UniversityPulsed-field gel electrophoresis Multilocus sequence typing PBP fingerprinting Pulsed-field gel electrophoresis (PFGE) Genomic DNA is prepared in situ in agarose blocks as described previously (1,2) and is digested with SmaI (Life Technologies,Gaithersburg Md).The fragments are resolved by PFGE in 1% agarose (SeaKem GTG agarose Comparison of Multilocus Sequence Typing and PulsedMultilocus sequence typing (MLST) of Staphylococcus aureus is well suited to the study of global or long-term epidemiology,but its role in local epidemiology has not been defined.The present study has compared MLST with pulsed-field gel electrophoresis (PFGE) by using S.aureus isolates associated with carriage and disease in a busy regional renal unit.

Comparison of Multilocus Sequence Typing and Pulsed

Multilocus sequence typing (MLST) of Staphylococcus aureus is well suited to the study of global or long-term epidemiology,but its role in local epidemiology has not been defined.The present study has compared MLST with pulsed-field gel electrophoresis (PFGE) by using S.aureus isolates associated with carriage and disease in a busy regional renal unit.Combination of multilocus sequence typing and pulsed Multilocus Sequence Typing Pulsed#0183;Multilocus sequence typing (MLST) is a useful molecular technique for assessing evolutionary and population biology relationships,especially tracing the evolution of epidemic clones,whereas pulsed-field gel electrophoresis (PFGE) has advantages for distinguishing closely-related strains within an epidemic clone or local epidemic clones Combination of multilocus sequence typing and pulsed Multilocus Sequence Typing Pulsed#0183;Multilocus sequence typing (MLST) is a useful molecular technique for assessing evolutionary and population biology relationships,especially tracing the evolution of epidemic clones,whereas pulsed-field gel electrophoresis (PFGE) has advantages for distinguishing closely-related strains within an epidemic clone or local epidemic clones

Cited by 87Publish Year 2005Author LL Nemoy,M Kotetishvili,J Tigno,A Keefer-Norris,AD Harris,EN Perencevich,JA Johnson,D Torpey,Comparison of Multilocus Sequence Typing,Pulsed-Field Gel

Although pulsed-field gel electrophoresis (PFGE) is the current gold standard typing method for Salmonella,multilocus sequence typing (MLST) may be more relevant to investigations exploring evolutionary and population biology relationships.Cited by 207Publish Year 2003Author Anna C.Noller,M.Catherine McEllistrem,O.Colin Stine,J.Glenn Morris,David J.Boxrud,Bruce DiPhylogenetic investigation of Edwardsiella tarda with Oct 10,2013 Multilocus Sequence Typing Pulsed#0183;Edwardsiella tarda is the etiologic agent of Edwardsiellosis,a devastating fish disease prevailing in worldwide aquaculture industries.Here we developed the SpeI-based pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) assays to investigate the genetic diversity of Edwardsiella isolates obtained from aquaculture farms and to get insights into theCited by 189Publish Year 2006Author H.Harbottle,D.G.White,P.F.McDermott,R.D.Walker,S.ZhaoMultilocus Sequence Typing Reveals a Lack of Diversity Feb 01,2003 Multilocus Sequence Typing Pulsed#0183;Escherichia coli O157:H7 is a major cause of foodborne illness in the United States.Pulsed-field gel electrophoresis (PFGE) is the molecular epidemiologic method mostly commonly used to identify food-borne outbreaks.Although PFGE is a powerful epidemiologic tool,it has disadvantages that make a DNA sequence-based approach potentially attractive.Multilocus sequence typing (MLST)

Cited by 154Publish Year 2005Author Mohamed K.Fakhr,Lisa K.Nolan,Catherine M.LogueMultilocus Sequence Typing versus Pulsed-Field Gel

Pulsed-field gel electrophoresis (PFGE) is the most widely used tool for molecular typing of bacterial strains (),but new DNA fingerprinting techniques,including multilocus sequence typing (MLST),are emerging as alternatives,particularly when information regarding evolutionary history is needed ().MLST is a relatively new technique where multiple genes (loci) are sequenced to measure Cited by 141Publish Year 2007Author Jennifer K.Johnson,Sonia M.Arduino,O.Colin Stine,Judith A.Johnson,Anthony D.HarrisMultilocus Sequence Typing,Pulsed-Field Gel Dec 15,2008 Multilocus Sequence Typing Pulsed#0183;A total of 208 isolates were typed by sequencing the fla short variable region,macrorestriction analysis resolved by pulsed-field gel electrophoresis (PFGE),and multilocus sequence typing (MLST).We observed a high diversity ofCited by 12Publish Year 2015Author Tao Wang,Hua Li,Hua Wang,Jing SuImages of Multilocus Sequence Typing Pulsed imagesMultilocus Sequence Typing Compared to Pulsed-Field Gel Sep 19,2007 Multilocus Sequence Typing Pulsed#0183;The present study compared the discriminatory abilities of pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) for 90 P.aeruginosa isolates obtained from cultures of perirectal surveillance swabs from patients in an intensive care unit.PFGE identified 85 distinct types and 76 distinct groups when similarity cutoffs

Cited by 12Publish Year 2014Author Gabriel Esquitini Machado,Cristianne Kayoko Matsumoto,Erica Chimara,Rafael da Silva Duarte,DenisMultilocus sequence typing and pulsed-field gel

Apr 16,2015 Multilocus Sequence Typing Pulsed#0183;The present study established a typing method with NotI-based pulsed-field gel electrophoresis (PFGE) and stress response gene schemed multilocus sequence typing (MLST) for 55 Oenococcus oeni strains isolated from six individual regions in China and two model strains PSU-1 (CP000411) and ATCC BAA-1163 (AAUV00000000).Seven stress response genes,cfa,clpL,clpP,Cited by 11Publish Year 2013Author Minjun Yang,Shuai Shao,Jingfan Xiao,Qiyao Wang,Yuanxing ZhangDiversity and Mobility of Integrative and Conjugative The diversity of the isolates included in this study was demonstrated by multilocus sequence typing for S.agalactiae and pulsed-field gel electrophoresis for S.dysgalactiae and S.uberis .Most of the erythromycin-resistant strains carry an ermB gene.Cited by 100Publish Year 2008Author Catherine Ragimbeau,Fran Multilocus Sequence Typing Pulsed#231;ois Schneider,Serge Losch,Jos Even,Jo Multilocus Sequence Typing Pulsed#235;l MossongMultilocus Sequence Typing Lacks the Discriminatory Multilocus sequence typing (MLST),a recently developed methodology that requires minimal human input,has also been used to type Salmonella strains .This technique is based on determination of the DNA sequence of a series of selected housekeeping,ribosomal,and/or virulence-associated genes (for reviews,see references 6 and 28).

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